SCIENTIFIC PUBLICATIONS

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Key Publications

• Schaaf TM, KC Peterson, BD Grant, P Bawaskar, S Yuen, J Li, JM Muretta, GD Gillispie, and DD Thomas. 2016. High-throughput spectral and lifetime-based FRET screening in living cells to identify small-molecule effectors of SERCA. J Biomol Screen ​(In Press).

Abstract

• Schaaf TM, KC Peterson, BD Grant, DD Thomas, and GD Gillispie. 2016. Spectral unmixing plate reader: high-throughput, high-precision FRET assays in living cells. J Biomol Screen (In Press).

Abstract

• Rebbeck, RT, MM Essawy, FR Nitu, BD Grant, GD Gillispie, DD Thomas, DM Bers, and RL Cornea. 2016. High-throughput screens to discover small molecule modulators of ryanodine receptor calcium release channels. J Biomol Screen (In Press). 

Abstract

• Oda, T, Y Yang, H Uchinoumi, DD Thomas, Y Chen-Izu, T Kato, T Yamamoto, M Yano, RL Cornea, and DM Bers. 2015. Oxidation of ryanodine receptor (RyR) and calmodulin enhance Ca release and pathologically alter, RyR structure and calmodulin affinity. J Molec Cell Cardiol 85:240-248. PMC4530019.

Abstract

• Petersen, KJ, KC Peterson, JM Muretta, SE Higgins, GD Gillispie, and DD Thomas. 2014. Fluorescence lifetime plate reader: resolution and precision meet high-throughput. Rev Sci Instrum 81:113101-113107. PMC4242087.

Abstract

• Gruber, SJ, RL Cornea, J Li, KC Peterson, TM Schaaf, GD Gillispie, R Dahl, KM Zsebo, SL Robia, and DD Thomas. 2014. Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells. J Biomol Screen 19:215-222 (cover article). PMC4013825.

Abstract

• Cornea, RL, SJ Gruber, EL Lockamy, JM Muretta, D Jin, J Chen, R Dahl, T Bartfai, KM Zsebo, GD Gillispie, and DD Thomas. 2013. High-throughput FRET assay yields allosteric SERCA activators. J Biomol Screen 18:97-107. PMC3721969.

Abstract

• Oda, T., Yang, Y., Nitu, F.R., Svensson, B., Lu, X., Fruen, B.R., Cornea, R.L., and Bers D.M (2013) Binding of RyR2 “Unzipping” Peptide in Cardiomyocytes Activates RyR2 and Reciprocally Inhibits Calmodulin Binding. Circ. Res. 112, 487-97. PMCID: PMC3573756.

Abstract

• Muretta, J.M., A. Kyrychenko, A.S. Ladokhin, D. Kast, G.E. Gillispie, and D.D. Thomas. 2010. High-performance time-resolved fluorescence by direct waveform recording. Rev Sci Instrum, 81: 103101 (1-8). PMC2980540.

Abstract

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Other Relevant Publications

• Iram, SH, SJ Gruber, ON Raguimova, DD Thomas, and SL Robia. 2015. ATP-binding cassette transporter structure changes detected by intramolecular fluorescence energy transfer for high-throughput screening. Mol Pharmacy, 88(1):84-94. PMCID: PMC4468642.

• Dong, X, and DD Thomas. 2014. Time-resolved FRET reveals the structural mechanism of SERCA-PLB regulation. Biochem Biophys Res Commun 449:196-201. PMC4054823.

• Pallikuth, S, D Blackwell, Z Hu, Z Hou, DT Zieman, B Svensson, DD Thomas, and SL Robia. 2013. Phosphorylated phospholamban stabilizes a compact conformation of the cardiac calcium-ATPase. Biophys J 105:1812-21. PMC3797577.

• Muretta, JM, KJ Petersen, and DD Thomas. 2013. Direct real-time detection of the actin-activated power stroke within the myosin catalytic domain. Proc Natl Acad Sci USA 110:7211–7216. PMC3645529.

• Hou, Z, S Hu, DJ Blackwell, TD Miller, DD Thomas, and SL Robia. 2012. Two-color calcium pump reveals closure of the cytoplasmic headpiece with calcium binding. PLoS One 7:e40369. PMC3394785.

• Valley, CC, JD Perlmutter, AR Braun, AK Lewis, CB Karim, DD Thomas, JR Brody, and JN Sachs. 2012. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces death receptor 5 networks that are highly organized. J Biol Chem 287:21265-21278. PMC3375548.

• Gruber, SJ, S Haydon, and DD Thomas. 2012. Phospholamban mutants compete with wild type for SERCA binding in HEK Cells. Biochem Biophys Res Commun 420(5): 236-240. PMC3324857.

• Li, J, ZM James, X Dong, CB Karim, and DD Thomas. 2012. Structural and functional dynamics of an integral membrane protein complex modulated by lipid headgroup charge. J Mol Biol. NIHMSID 361218. PMC3327772.

• Nesmelov, YE, RV Agafonov, IV Negrashov, SE Blakely, MA Titus, and DD Thomas. 2011. Structural kinetics of myosin by transient time-resolved FRET. Proc Nat Acad Sci USA, 108:1891–1896. PMC3033248 

• Cornea, R.L., F. Nitu, S. Gruber, K. Kohler, M. Satzer, D.D. Thomas, and B.R. Fruen. 2009. FRET-based mapping of calmodulin bound to the RyR1 Ca2+ release channel. Proc Natl Acad Sci U S A. 106: 6128-6133. PMC2662960.

• Winters, D.L., J.M. Autry, B. Svensson, and D.D. Thomas. 2008. Interdomain FRET in SERCA probed by cyan-fluorescent protein fused to the actuator domain. Biochemistry. 47: 4246-4256.