SCIENTIFIC PUBLICATIONS

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Key Publications

• Stroik, DR, SL Yuen, KA Janicek, TM Schaaf, J Li, DK Ceholski, RJ Hajjar, RL Cornea, and DD Thomas. 2018. Targeting protein-protein interactions for therapeutic discovery via FRET-based high-throughput screening in living cells. Nature Sci Rep 8:12560. 

Abstract

• Guhathakurta, P, E Prochniewicz, BD Grant, KC Peterson, and DD Thomas. 2018. High-throughput screen, using time-resolved FRET, yields actin-binding components that modulate actin-myosin structure and function. J Biol Chem 293:12288-12298. 

Abstract

• Lo, CH, N Vunnam, AK Lewis, TL Chiu, BE Brummel, TM Schaaf, BD Grant, P Bawaskar, DD Thomas, and JN Sachs. 2017. An innovative high-throughput screening approach for discovery of small molecules that inhibit TNF Receptors. SLAS Discov 22(8):950-961.

Abstract

• Schaaf TM, KC Peterson, BD Grant, P Bawaskar, S Yuen, J Li, JM Muretta, GD Gillispie, and DD Thomas. 2017. High-throughput spectral and lifetime-based FRET screening in living cells to identify small-molecule effectors of SERCA. SLAS Discov 22:262-273.

Abstract

• Schaaf TM, KC Peterson, BD Grant, DD Thomas, and GD Gillispie. 2017. Spectral unmixing plate reader: high-throughput, high-precision FRET assays in living cells. ​SLAS Discov 22:250-261.

Abstract

• Rebbeck, RT, MM Essawy, FR Nitu, BD Grant, GD Gillispie, DD Thomas, DM Bers, and RL Cornea. 2017. High-throughput screens to discover small molecule modulators of ryanodine receptor calcium release channels. SLAS Discov 22(2):176-186. 

Abstract

• Oda, T, Y Yang, H Uchinoumi, DD Thomas, Y Chen-Izu, T Kato, T Yamamoto, M Yano, RL Cornea, and DM Bers. 2015. Oxidation of ryanodine receptor (RyR) and calmodulin enhance Ca release and pathologically alter RyR structure and calmodulin affinity. J Mol Cell Cardiol 85:240-248. 

Abstract

• Petersen, KJ, KC Peterson, JM Muretta, SE Higgins, GD Gillispie, and DD Thomas. 2014. Fluorescence lifetime plate reader: resolution and precision meet high-throughput. Rev Sci Instrum 85:113101-113107. 

Abstract

• Gruber, SJ, RL Cornea, J Li, KC Peterson, TM Schaaf, GD Gillispie, R Dahl, KM Zsebo, SL Robia, and DD Thomas. 2014. Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells. J Biomol Screen 19:215-222.

Abstract

• Cornea, RL, SJ Gruber, EL Lockamy, JM Muretta, D Jin, J Chen, R Dahl, T Bartfai, KM Zsebo, GD Gillispie, and DD Thomas. 2012. High-throughput FRET assay yields allosteric SERCA activators. J Biomol Screen 18:97-107.

Abstract

• Oda, T, Y Yang, FR Nitu, B Svensson, X Lu, BR Fruen, RL Cornea, and DM Bers. 2013. In cardiomyocytes, binding of unzipping peptide activates ryanodine receptor 2 and reciprocally inhibits calmodulin binding. Circulation Research 112(3):487-97.

Abstract

• Muretta, JM, A Kyrychenko, AS Ladokhin, D Kast, GE Gillispie, and DD Thomas. 2010. High-performance time-resolved fluorescence by direct waveform recording. Rev Sci Instrum, 81:103101 (1-8). 

Abstract

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Other Relevant Publications

• Guhathakurta, P, E Prochniewicz, and DD Thomas. 2018. Actin-myosin interaction: structure, function, and drug discovery. Int J Mol Sci 19:2628
• Rohde, JA, DD Thomas, and JM Muretta. 2017. Heart failure drug changes the mechanoenzymology of the cardiac myosin power stroke. Proc Natl Acad Sci U S A 114:E1796-E1804.
• Uchinoumi, H, Y Yang, T Oda, N Li, KM Alsina, JL Puglisi, Y Chen-Izu, RL Cornea, XHT Wehrens, DM Bers. 2016. CamKII-dependent phosphorylation of RyR2 promotes targetable pathological RyR2 conformational shift. J Mol Cell Cardiol 98:62-72.
  
• Kraft, TE, MR Heitmeier, M Putanko, RL Edward, MXG Ilagan, MA Payne, JM Autry, DD Thomas, AR Odom, and PW Hruz. 2016. A novel fluorescence resonance energy transfer-based screen in high-throughput format to identify inhibitors of malarial and human glucose transporters. Antimicrob Agents Chemother 60:7007-7014.
• Iram, SH, SJ Gruber, ON Raguimova, DD Thomas, and SL Robia. 2015. ATP-binding cassette transporter structure changes detected by intramolecular fluorescence energy transfer for high-throughput screening. Mol Pharmacy, 88:84-94. 

• Dong, X, and DD Thomas. 2014. Time-resolved FRET reveals the structural mechanism of SERCA-PLB regulation. Biochem Biophys Res Commun 449:196-201. 

• Pallikuth, S, D Blackwell, Z Hu, Z Hou, DT Zieman, B Svensson, DD Thomas, and SL Robia. 2013. Phosphorylated phospholamban stabilizes a compact conformation of the cardiac calcium-ATPase. Biophys J 105:1812-21. 

• Muretta, JM, KJ Petersen, and DD Thomas. 2013. Direct real-time detection of the actin-activated power stroke within the myosin catalytic domain. Proc Natl Acad Sci U S A 110:7211–7216. 

• Hou, Z, S Hu, DJ Blackwell, TD Miller, DD Thomas, and SL Robia. 2012. Two-color calcium pump reveals closure of the cytoplasmic headpiece with calcium binding. PLoS One 7:e40369.

• Valley, CC, JD Perlmutter, AR Braun, AK Lewis, CB Karim, DD Thomas, JR Brody, and JN Sachs. 2012. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces death receptor 5 networks that are highly organized. J Biol Chem 287:21265-21278.

• Gruber, SJ, S Haydon, and DD Thomas. 2012. Phospholamban mutants compete with wild type for SERCA binding in living cells. Biochem Biophys Res Commun 420: 236-240. 

• Li, J, ZM James, X Dong, CB Karim, and DD Thomas. 2012. Structural and functional dynamics of an integral membrane protein complex modulated by lipid headgroup charge. J Mol Biol 418:379-389

• Nesmelov, YE, RV Agafonov, IV Negrashov, SE Blakely, MA Titus, and DD Thomas. 2011. Structural kinetics of myosin by transient time-resolved FRET. Proc Nat Acad Sci USA, 108:1891–1896. 

• Cornea, RL, F Nitu, S Gruber, K Kohler, M Satzer, DD Thomas, and BR Fruen. 2009. FRET-based mapping of calmodulin bound to the RyR1 Ca2+ release channel. Proc Natl Acad Sci U S A. 106: 6128-6133. 

• Winters, DL, JM Autry, B Svensson, and DD Thomas. 2008. Interdomain fluorescence resonance energy transfer in SERCA probed by cyan fluorescent protein fused to the actuator domain. Biochemistry. 47:4246-4256.