Razvan L. Cornea Publications & Patents
University of Minnesota – Department of Biochemistry, Molecular Biology and Biophysics
6-155 JacH, 321 Church Street SE, Minneapolis, MN 55455
Phone: (612) 626-2660; Fax: (612) 624-5121;
E-mail: Razvan.Cornea@PhotonicPharma.com
http://www.cbs.umn.edu/bmbb/contacts/razvan-l-cornea
Peer-Reviewed Publications
1. Cornea, R.L., & Thomas, D.D. (1994) Effects of Membrane Thickness on the Molecular Dynamics and Enzymatic Activity of Reconstituted Sarcoplasmic Reticulum Ca-ATPase. Biochemistry 33, 2912-2920, PMID: 8130205.
2. Cornea, R.L., L.R. Jones, J.M. Autry, and D.D. Thomas. (1997) Mutation and phosphorylation change the oligomeric structure of phospholamban in lipid bilayers. Biochemistry 36, 2960-2967, 9062126.
3. Li, M., R.L. Cornea, J.M. Autry, L.R. Jones, and D.D. Thomas. (1998) Phosphorylation-Induced Structural Change in Phospholamban and Its Mutants, Detected by Intrinsic Fluorescence. Biochemistry 37, 7869-7877, PMID: 9601048.
4. Thomas, D.D., L.G. Reddy, C.B. Karim, M. Li, R.L. Cornea, and J. Stamm. (1998) Direct Spectroscopic Detection of Molecular Dynamics and Interactions of the Calcium Pump and Phospholamban. Annals of the NYAS 853: 186-194.
5. Cornea, R.L., J.M. Autry, Z. Chen, and L.R. Jones. (2000) Re-examination of the Role of the Leucine/Isoleucine Zipper Residues of Phospholamban in Inhibition of the Ca2+-Pump of Cardiac Sarcoplasmic Reticulum. J. Biol. Chem. 275, 41487-41494, PMID: 11016944.
6. Jones, L.R., R.L. Cornea, and Z. Chen. (2002) Close proximity between residue 30 of phospholamban and cysteine 318 of the cardiac Ca2+-pump revealed by intermolecular thiol cross-linking. J. Biol. Chem. 277, 28319-28329, PMID: 12015326.
7. Balog, E.M., L.E. Norton, R.A. Bloomquist, R.L. Cornea, D.J. Black, C.F. Louis, D.D. Thomas, and B.R. Freud. (2003) Calmodulin oxidation and methionine to glutamine substitutions reveal methionine residues critical for functional interaction with RyR1. J. Biol. Chem. 278, 15615-15621, PMID:12586832.
8. Reddy, L.G., R.L. Cornea, D.L. Winters, E. McKenna, and D.D. Thomas. (2003) Defining the molecular components of Ca-transport regulation in a reconstituted membrane system. Biochemistry 42, 4585-4592, PMID: 12693956.
9. Fruen, B.R., E.M. Balog, J. Schafer, F.R. Nitu, D.D. Thomas, and R.L. Cornea. (2005) Direct detection of calmodulin tuning by ryanodine receptor channel targets using a Ca2+-sensitive acrylodan-labeled calmodulin. Biochemistry 44, 278-284, PMID: 15628869.
10. Cornea, R.L., F.R. Nitu, S. Gruber, K. Kohler, M. Satzer, D.D. Thomas, and B.R. Fruen. (2009) FRET-based mapping of calmodulin bound to the RyR1 Ca2+ release channel. Proc Natl Acad Sci USA 106, 6128-6133, PMCID: 2662960.
11. Guo, T.*, R.L. Cornea*, S. Huke, E. Camors, Y. Yang, E. Picht, B.R. Fruen, and D.M. Bers. (2010) Kinetics of FKBP12.6 binding to ryanodine receptors in permeabilized cardiac myocytes and effects on Ca sparks. Circ. Res. Cover article 106, 1743-1752; PMCID: PMC2895429. *Co-first authors.
12. Cornea, R.L., F.R. Nitu, M. Samsó, D.D. Thomas, and B.R. Fruen. (2010) Mapping The Ryanodine Receptor (RyR) FK506-Binding Protein (FKBP) Subunit Using Fluorescence Resonance Energy Transfer (FRET). J. Biol. Chem. 285, 19219-26. PMCID: PMC2885200.
13. Lockamy, E.L., R.L. Cornea, C.B. Karim, and D.D. Thomas. (2011) Functional and Physical Competition between Phospholamban and its Mutants Provides Insight into the Molecular Mechanism of Gene Therapy for Heart Failure. Biochem. Biophys. Res. Commun. 408,388-92. PMCID: PMC3212041.
14. Guo, T., B.R. Fruen, F.R. Nitu, T.D. Nguyen, Y. Yang, R.L. Cornea, and D.M. Bers. (2011) FRET Detection of Calmodulin Binding to the Cardiac RyR2 Calcium Release Channel. Biophys. J. 101, 2170-77. PMCID: PMC3207159.
15. Cornea, R.L., S.J. Gruber, E.L. Lockamy, J.M. Muretta, D. Jin, J. Chen, R. Dahl, T. Bartfai, K.M. Zsebo, G.D. Gillispie, and D.D. Thomas. (2013) High-Throughput FRET Assay Yields Allosteric SERCA Activators. J. Biomol. Screen. 18, 96-106. PMCID:PMC3721969.
16. Oda, T., Y. Yang, F.R. Nitu, B. Svensson, X. Lu, B.R. Fruen, R.L. Cornea, and D.M. Bers. (2013) Binding of RyR2 “Unzipping” Peptide in Cardiomyocytes Activates RyR2 and Reciprocally Inhibits Calmodulin Binding. Circ. Res. 112, 487-97. PMCID: PMC3573756.
17. Girgenrath, T., M. Mahalingam, B. Svensson, F.R. Nitu, R.L. Cornea, and J.D. Fessenden. (2013) N-terminal and Central Segments of the Type 1 Ryanodine Receptor (RyR1) Mediate Its Interaction with FK506 Binding Proteins. J. Biol. Chem. 288,16073-84. PMCID: PMC3668763.
18. Bleeker, N.P., R.L. Cornea, D.D. Thomas, and C. Xing. (2013) A novel SERCA inhibitor demonstrates synergy with classic SERCA inhibitors and targets multi-drug resistant AML. Mol. Pharmaceutics. 10, 4358-66. PMCID:PMC3946399.
19. Gruber, S.J., R.L. Cornea, J. Li, K.C. Peterson, G.D. Gillispie, R. Dahl, K.M. Zsebo, S.L. Robin, and D.D. Thomas. (2014) Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells. J Biomol Screen. Cover article 19, 215-22 PMCID: PMC4013825
20. Yang, Y., T. Guo, T. Oda, A. Chakraborty, L. Chen, A.A. Knowlton, B.R. Fruen, R.L. Cornea, G. Meissner, and D.M. Bers. (2014) In cardiac myocytes, Z-Line calmodulin is mainly RyR2-bound and heart failure decreases RyR2-CaM affinity. Circ Res. 114, 295-306.
21. Hwang, H.S., F.R. Nitu, Y. Yang, K. Walweel, L. Pereira, C.N. Johnson, M. Faggioni, W.J. Chazin, D. Laver, A.L. George, R.L. Cornea, D.M. Bers, and B.C. Knollmann. (2014) Divergent Regulation of RyR2 Channels by Arrhythmogenic Human Calmodulin Mutants. Circ Res. 114, 1114-24. PMCID:PMC3990285.
22. Mahalingam,M., T. Girgenrath, B. Svensson, D.D. Thomas, R.L. Cornea,* and J.D. Fessenden* . (2014) Structural Mapping of Divergent Regions in the Type 1 Ryanodine Receptor Using Fluorescence Resonance Energy Transfer. Structure 22:1322-1332 (*co-senior authors).
23. Svensson, B., T. Oda, F.R. Nitu, Y. Yang, I. Cornea, Y. Chen-Izu, J.D. Fessenden, D.M. Bers, D.D. Thomas, and R.L. Cornea. (2014) FRET-Based Trilateration of Probes Bound within Functional Ryanodine Receptors. Biophys. J cover article. 107:2037-2048.
24. Oda, T., Y. Yang, H.,Uchinoumi, D.D. Thomas, Y. Chen-Izu, T. Kato, T. Yamamoto, M. Yano, R.L. Cornea, and D.M. Bers. (2015) Oxidation of Ryanodine Receptor (RyR) and Calmodulin Enhance Ca Release and Pathologically Alter RyR Structure and Calmodulin Affinity. J Mol Cel Cardiol. 85:240-248.
25. Rebbeck, R., F.R. Nitu, D. Rohde, P. Most, D.M. Bers, D.D. Thomas, and R.L. Cornea. (2016) S100A1 does not compete with calmodulin for ryanodine receptor binding but structurally alters the ryanodine receptor/calmodulin complex. J Biol Chem in press.
Patents
U.S. patent 8,431,356: Fluorescence resonance energy transfer assays for sarco/endoplasmic reticulum calcium ATPase and phospholamban. Inventors: David D. Thomas, Razvan L. Cornea, and Krisztina M. Zsebo.
U.S. patent application no. 14/565,811: FRET-based high-throughput screening methods to discover modulators of RyR calcium channels (other inventors Donald Bers, David Thomas, Gregory Gillispie). Filed Dec.10, 2014.
U.S. patent application no. 14/598,707: High-throughput, high-precision methods for detecting protein structural changes in living cells (other inventors, David Thomas, Simon Gruber, Gregory Gillispie, Seth Robia). Filed Jan. 16, 2015.
6-155 JacH, 321 Church Street SE, Minneapolis, MN 55455
Phone: (612) 626-2660; Fax: (612) 624-5121;
E-mail: Razvan.Cornea@PhotonicPharma.com
http://www.cbs.umn.edu/bmbb/contacts/razvan-l-cornea
Peer-Reviewed Publications
1. Cornea, R.L., & Thomas, D.D. (1994) Effects of Membrane Thickness on the Molecular Dynamics and Enzymatic Activity of Reconstituted Sarcoplasmic Reticulum Ca-ATPase. Biochemistry 33, 2912-2920, PMID: 8130205.
2. Cornea, R.L., L.R. Jones, J.M. Autry, and D.D. Thomas. (1997) Mutation and phosphorylation change the oligomeric structure of phospholamban in lipid bilayers. Biochemistry 36, 2960-2967, 9062126.
3. Li, M., R.L. Cornea, J.M. Autry, L.R. Jones, and D.D. Thomas. (1998) Phosphorylation-Induced Structural Change in Phospholamban and Its Mutants, Detected by Intrinsic Fluorescence. Biochemistry 37, 7869-7877, PMID: 9601048.
4. Thomas, D.D., L.G. Reddy, C.B. Karim, M. Li, R.L. Cornea, and J. Stamm. (1998) Direct Spectroscopic Detection of Molecular Dynamics and Interactions of the Calcium Pump and Phospholamban. Annals of the NYAS 853: 186-194.
5. Cornea, R.L., J.M. Autry, Z. Chen, and L.R. Jones. (2000) Re-examination of the Role of the Leucine/Isoleucine Zipper Residues of Phospholamban in Inhibition of the Ca2+-Pump of Cardiac Sarcoplasmic Reticulum. J. Biol. Chem. 275, 41487-41494, PMID: 11016944.
6. Jones, L.R., R.L. Cornea, and Z. Chen. (2002) Close proximity between residue 30 of phospholamban and cysteine 318 of the cardiac Ca2+-pump revealed by intermolecular thiol cross-linking. J. Biol. Chem. 277, 28319-28329, PMID: 12015326.
7. Balog, E.M., L.E. Norton, R.A. Bloomquist, R.L. Cornea, D.J. Black, C.F. Louis, D.D. Thomas, and B.R. Freud. (2003) Calmodulin oxidation and methionine to glutamine substitutions reveal methionine residues critical for functional interaction with RyR1. J. Biol. Chem. 278, 15615-15621, PMID:12586832.
8. Reddy, L.G., R.L. Cornea, D.L. Winters, E. McKenna, and D.D. Thomas. (2003) Defining the molecular components of Ca-transport regulation in a reconstituted membrane system. Biochemistry 42, 4585-4592, PMID: 12693956.
9. Fruen, B.R., E.M. Balog, J. Schafer, F.R. Nitu, D.D. Thomas, and R.L. Cornea. (2005) Direct detection of calmodulin tuning by ryanodine receptor channel targets using a Ca2+-sensitive acrylodan-labeled calmodulin. Biochemistry 44, 278-284, PMID: 15628869.
10. Cornea, R.L., F.R. Nitu, S. Gruber, K. Kohler, M. Satzer, D.D. Thomas, and B.R. Fruen. (2009) FRET-based mapping of calmodulin bound to the RyR1 Ca2+ release channel. Proc Natl Acad Sci USA 106, 6128-6133, PMCID: 2662960.
11. Guo, T.*, R.L. Cornea*, S. Huke, E. Camors, Y. Yang, E. Picht, B.R. Fruen, and D.M. Bers. (2010) Kinetics of FKBP12.6 binding to ryanodine receptors in permeabilized cardiac myocytes and effects on Ca sparks. Circ. Res. Cover article 106, 1743-1752; PMCID: PMC2895429. *Co-first authors.
12. Cornea, R.L., F.R. Nitu, M. Samsó, D.D. Thomas, and B.R. Fruen. (2010) Mapping The Ryanodine Receptor (RyR) FK506-Binding Protein (FKBP) Subunit Using Fluorescence Resonance Energy Transfer (FRET). J. Biol. Chem. 285, 19219-26. PMCID: PMC2885200.
13. Lockamy, E.L., R.L. Cornea, C.B. Karim, and D.D. Thomas. (2011) Functional and Physical Competition between Phospholamban and its Mutants Provides Insight into the Molecular Mechanism of Gene Therapy for Heart Failure. Biochem. Biophys. Res. Commun. 408,388-92. PMCID: PMC3212041.
14. Guo, T., B.R. Fruen, F.R. Nitu, T.D. Nguyen, Y. Yang, R.L. Cornea, and D.M. Bers. (2011) FRET Detection of Calmodulin Binding to the Cardiac RyR2 Calcium Release Channel. Biophys. J. 101, 2170-77. PMCID: PMC3207159.
15. Cornea, R.L., S.J. Gruber, E.L. Lockamy, J.M. Muretta, D. Jin, J. Chen, R. Dahl, T. Bartfai, K.M. Zsebo, G.D. Gillispie, and D.D. Thomas. (2013) High-Throughput FRET Assay Yields Allosteric SERCA Activators. J. Biomol. Screen. 18, 96-106. PMCID:PMC3721969.
16. Oda, T., Y. Yang, F.R. Nitu, B. Svensson, X. Lu, B.R. Fruen, R.L. Cornea, and D.M. Bers. (2013) Binding of RyR2 “Unzipping” Peptide in Cardiomyocytes Activates RyR2 and Reciprocally Inhibits Calmodulin Binding. Circ. Res. 112, 487-97. PMCID: PMC3573756.
17. Girgenrath, T., M. Mahalingam, B. Svensson, F.R. Nitu, R.L. Cornea, and J.D. Fessenden. (2013) N-terminal and Central Segments of the Type 1 Ryanodine Receptor (RyR1) Mediate Its Interaction with FK506 Binding Proteins. J. Biol. Chem. 288,16073-84. PMCID: PMC3668763.
18. Bleeker, N.P., R.L. Cornea, D.D. Thomas, and C. Xing. (2013) A novel SERCA inhibitor demonstrates synergy with classic SERCA inhibitors and targets multi-drug resistant AML. Mol. Pharmaceutics. 10, 4358-66. PMCID:PMC3946399.
19. Gruber, S.J., R.L. Cornea, J. Li, K.C. Peterson, G.D. Gillispie, R. Dahl, K.M. Zsebo, S.L. Robin, and D.D. Thomas. (2014) Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells. J Biomol Screen. Cover article 19, 215-22 PMCID: PMC4013825
20. Yang, Y., T. Guo, T. Oda, A. Chakraborty, L. Chen, A.A. Knowlton, B.R. Fruen, R.L. Cornea, G. Meissner, and D.M. Bers. (2014) In cardiac myocytes, Z-Line calmodulin is mainly RyR2-bound and heart failure decreases RyR2-CaM affinity. Circ Res. 114, 295-306.
21. Hwang, H.S., F.R. Nitu, Y. Yang, K. Walweel, L. Pereira, C.N. Johnson, M. Faggioni, W.J. Chazin, D. Laver, A.L. George, R.L. Cornea, D.M. Bers, and B.C. Knollmann. (2014) Divergent Regulation of RyR2 Channels by Arrhythmogenic Human Calmodulin Mutants. Circ Res. 114, 1114-24. PMCID:PMC3990285.
22. Mahalingam,M., T. Girgenrath, B. Svensson, D.D. Thomas, R.L. Cornea,* and J.D. Fessenden* . (2014) Structural Mapping of Divergent Regions in the Type 1 Ryanodine Receptor Using Fluorescence Resonance Energy Transfer. Structure 22:1322-1332 (*co-senior authors).
23. Svensson, B., T. Oda, F.R. Nitu, Y. Yang, I. Cornea, Y. Chen-Izu, J.D. Fessenden, D.M. Bers, D.D. Thomas, and R.L. Cornea. (2014) FRET-Based Trilateration of Probes Bound within Functional Ryanodine Receptors. Biophys. J cover article. 107:2037-2048.
24. Oda, T., Y. Yang, H.,Uchinoumi, D.D. Thomas, Y. Chen-Izu, T. Kato, T. Yamamoto, M. Yano, R.L. Cornea, and D.M. Bers. (2015) Oxidation of Ryanodine Receptor (RyR) and Calmodulin Enhance Ca Release and Pathologically Alter RyR Structure and Calmodulin Affinity. J Mol Cel Cardiol. 85:240-248.
25. Rebbeck, R., F.R. Nitu, D. Rohde, P. Most, D.M. Bers, D.D. Thomas, and R.L. Cornea. (2016) S100A1 does not compete with calmodulin for ryanodine receptor binding but structurally alters the ryanodine receptor/calmodulin complex. J Biol Chem in press.
Patents
U.S. patent 8,431,356: Fluorescence resonance energy transfer assays for sarco/endoplasmic reticulum calcium ATPase and phospholamban. Inventors: David D. Thomas, Razvan L. Cornea, and Krisztina M. Zsebo.
U.S. patent application no. 14/565,811: FRET-based high-throughput screening methods to discover modulators of RyR calcium channels (other inventors Donald Bers, David Thomas, Gregory Gillispie). Filed Dec.10, 2014.
U.S. patent application no. 14/598,707: High-throughput, high-precision methods for detecting protein structural changes in living cells (other inventors, David Thomas, Simon Gruber, Gregory Gillispie, Seth Robia). Filed Jan. 16, 2015.